Isolation and molecular identification of moderate halophilic enzyme-producing bacteria

Hassan, Gamal; Abdelal, Gehad; Hemeda, Nada; Eissa, Eissa

doi:10.21608/ifjsis.2024.307199.1084

Isolation and molecular identification of moderate halophilic enzyme-producing bacteria

Articles in Press

Document Type : Original full papers (regular papers)

Authors

¹ Genetics department, Faulty of Agriculture, Fayoum University, Egypt

² Department of Genetics, Faculty of Agriculture, Fayoum University, Egypt,

³ GenetDepartment of Genetics, Faculty of Agriculture, Fayoum University, Egyptics department, Faculty of Agriculture, Fayoum University , Egypt

⁴ Department of Genetics, Faculty of Agriculture, Fayoum University, Egypt

10.21608/ifjsis.2024.307199.1084

Abstract

The production of industrially relevant monomeric subunits from the hydrolysis of 1-4linkages in cellulose is catalyzed by cellulase applications for cellulases can be found in the food and feed textile brewing washing and pulp and paper industries the need for cellulases that can endure extreme circumstances related to pH, temperature and salinity is constant in these sectors. Thus, newly identified halophilic bacteria that produce halotolerant cellulose was presented in this study. cellulase 10 isolates of moderate halophiles bacteria were isolated from different samples collected from Qarun lake Fayoum Egypt. However, only five isolates S1, S2, S3, S4 and S5 showed highest cellulolytic activity were chosen for further analysis. The optimum conditions of maximum cellulase production were following 35ºC, pH 9, 15 NaCl and 3 days culture age respectively. The isolates exhibiting the greatest level of cellulase activity ranged in enzyme activity from 1.039 to 1.398 U/ml. It came to light that the majority of the halophilic isolates were Gram-negative and that their coloring varied from creamy white to yellow. Based on their 16S rRNA gene sequences, the S4 isolate was identified as Halomonas eurihaalin.

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